MRemondes Lab - Brain Maps and Decision-making
  • The MRemondes Lab
  • Research
  • Publications
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  • Anatomy
  • In vitro electrophysiology
  • In vivo electrophysiology
  • Putting it all toguether - electrophysiology and neural manipulation during behavior
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in vitro electrophysiology




Once identified, anatomical connections between brain regions of interest are tested for the possibility of establishing functional bona fide synapses supporting communication between such regions.
For this we combine optogenetics with multi-electrode electrophysiology using a Multi-electrode Array (MEA) with 60 channels distributed across region layers.
Picture
Injection of an anterograde virus in HIPP will express hChR2, a cation channel sensitive to blue light, in cortical targets. Fresh cortical slices are then laid on a MEA to record neural activity while we stimulate hCrR2-expressing HIPP axonal terminals targeting cortical layers, to study its anatomical distribution, and pharmacological sensitivity to blockers of the major synaptic conductances (AMPA, NMDA, GABAa).

Picture
HIPP axons stimulate preferentially deep ACC and superficial RSC layers in vitro.
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  • The MRemondes Lab
  • Research
  • Publications
  • Projects
  • Anatomy
  • In vitro electrophysiology
  • In vivo electrophysiology
  • Putting it all toguether - electrophysiology and neural manipulation during behavior
  • People
  • Map and Contacts
  • Useful Info/ Working at the MRemondesLab